Proteases of Dermatophagoides pteronyssinus
نویسندگان
چکیده
Since the discovery that Der p 1 is a cysteine protease, the role of proteolytic activity in allergic sensitization has been explored. There are many allergens with proteolytic activity; however, exposure from dust mites is not limited to allergens. In this paper, genomic, transcriptomic and proteomic data on Dermatophagoides pteronyssinus (DP) was mined for information regarding the complete degradome of this house dust mite. D. pteronyssinus has more proteases than the closely related Acari, Dermatophagoides farinae (DF) and Sarcoptes scabiei (SS). The group of proteases in D. pteronyssinus is found to be more highly transcribed than the norm for this species. The distribution of protease types is dominated by the cysteine proteases like Der p 1 that account for about half of protease transcription by abundance, and Der p 1 in particular accounts for 22% of the total protease transcripts. In an analysis of protease stability, the group of allergens (Der p 1, Der p 3, Der p 6, and Der p 9) is found to be more stable than the mean. It is also statistically demonstrated that the protease allergens are simultaneously more highly expressed and more stable than the group of D. pteronyssinus proteases being examined, consistent with common assumptions about allergens in general. There are several significant non-allergen outliers from the normal group of proteases with high expression and high stability that should be examined for IgE binding. This paper compiles the first holistic picture of the D. pteronyssinus degradome to which humans may be exposed.
منابع مشابه
Dermatophagoides pteronyssinus and bioelectric properties of airway epithelium: role of cysteine proteases.
Several epidemiological studies suggest that exposure to house dust mite allergens plays a role in the pathogenesis of asthma. Since many of these allergens exhibit enzymatic properties, they may damage the airway epithelium. To characterize the effects of low doses of Dermatophagoides pteronyssinus on the airway epithelium, the effect of D. pteronyssinus on the epithelial bioelectric propertie...
متن کاملSequence analysis of cDNA coding for a major house dust mite allergen, Der p 1. Homology with cysteine proteases
A cDNA clone coding for Der p 1, a major allergen from the house dust mite Dermatophagoides pteronyssinus, has been sequenced. It codes for a 222 residue mature protein with a derived molecular weight of 25,371 and contains 1 potential N-glycosylation site. In addition, the cDNA appears to code for a 13 residue proregion, and an incomplete signal peptide. The deduced sequence shows a high degre...
متن کاملLipid-binding allergens from Dermatophagoides pteronyssinus mites extract isolated by liposomes
Background House dust mites (HDM), including Dermatophagoides pteronyssinus (Dpt) and Dermatophagoides farinae (Df), are one of the commonest aeroallergens worldwide eliciting allergic manifestations [1]. Allergenic phenomenon is tightly associated both with the mites themselves and with ligands derived from mite-associated bacterial/fungal products. Some Dpt allergens belong of lipid-binding p...
متن کاملCorrelation between skin tests to Dermatophagoides pteronyssinus, Dermatophagoides siboney and Blomia tropicalis in Cuban asthmatics.
BACKGROUND Dermatophagoides pteronyssinus, Dermatophagoides siboney and Blomia tropicalis are the most important allergenic mites in Cuba. The aim of this study was to determine the degree of polysensitization and correlation of the skin prick test (SPT) reaction size to these mites in asthmatic patients. METHODS A total of 232 adult patients with asthmatic symptoms caused by house dust and p...
متن کاملSkin Prick Test Reactivity to Common Aero and Food Allergens among Children with Allergy
Background: The prevalence of allergic diseases has risen in the last decades. The objective of this study was to determine the common allergens in children via the skin prick test.Methods: This cross-sectional study recruited 313 allergic children (4 months to 18 years old) referred to the Asthma and Allergy Clinic of Children’s Medical Center in Tehran. A questionnaire containing demographic ...
متن کامل